Visitor of the Week: Allan Kalungi

Meet Allan Kalungi! The Ugandan national is affiliated with the Department of Psychiatry at Makerere University (Uganda) and the African Computational Genomics Group at MRC/UVRI and LSHTM Uganda Research Unit. The postdoctoral researcher joined at us last month’s workshop on Schizophrenia & Related Disorders—his first course at CSHL.

Tell us about your research.
My research is focused on understanding the genetic, biological and environmental factors that underly major and complex psychiatric disorders like depression among African populations from Africa.

How did you decide to focus on this area/project?
The underrepresentation of Africans in global psychiatric genetics research compelled me to focus on this area. Psychiatric disorders are a growing problem in Africa yet we do not know any of the genes or biological pathways for any of these disorders among populations from Africa, despite recent advances in psychiatric genetics research where the genetic nature of several psychiatric disorder has been illuminated. There is an urgent need to include Africans in global psychiatric genetics research if they are to benefit from recent psychiatric genetics discoveries.

What and/or who is the inspiration behind your scientific journey?
Africa had generally been left out in psychiatric genetics research. The current global attention to increase the visibility of Africans in global psychiatric research is a big inspiration in my scientific journey. In 2017, I pioneered psychiatric genetics research in East Africa when I produced the first article in the field that reported the association of selected serotonin transporter gene polymorphisms with increased suicidal risk among Uganda adults living with HIV.

Allan hiding in a cactus “forest” somewhere in Cape Town.

What impact do you hope to make through your work?
I hope my work will contribute to the understanding of the biology that underlies various psychiatric disorders among populations from Africa. I also hope that my work will refine the global understanding of the biology behind these disorders with the inclusion of the highly diverse genetics data from Africa.

Where do you see yourself in five years?
I see myself as an independent researcher on the forefront of psychiatric genetics research in Africa.

What do you love most about being a researcher?
I love being in an environment where things are not static and your thinking is under stimulation.

What drew you to apply to this course?
I am interested in understanding the nature of the comorbidity between depression and schizophrenia. My desire to understand the phenotypic and genetic nature of schizophrenia and its related disorders compelled me to apply to the course.

What is your key takeaway from the Course; and how do you plan to apply it to your work?
It is crucial to understand mechanisms that link genetic variations to disease. I plan to investigate these as well in my genetics studies.

What feedback or advice would you share with someone considering to participate in this course?
This is a great course where you will most likely catch up on recent, worldwide breakthroughs in all aspects of schizophrenia research; from Neuroscience approaches to Genetics/Epigenetics and Neuroimmunology.

Enjoying his last dinner of the course, and his first lobster!

What’s the most memorable thing that happened during the Course?
Meeting with top investigators in schizophrenia research like Robin Murray, Jeremy Hall, Anissa Abi-Dargham and James Walters among others. The most memorable moment was however the schizophrenia case presentation.

What do you like most about your time at CSHL?
I like the serenity around CSHL. It is isolated with no neighborhoods and is surrounded by trees. I enjoyed the breeze from the ocean. The food was nice and I enjoyed the lobster for the first time in my life!

Allan received a scholarship from the Howard Hughes Medical Institute (HHMI) to cover his course tuition. On behalf of Allan, thank you to HHMI for supporting and enabling our young scientists to attend a CSHL course where they expand their skills, knowledge, and network.

Thank you to Allan for being this week's featured visitor. To meet other featured scientists - and discover the wide range of science that takes part in a CSHL meeting or course - go here.

Images provided by Allan Kalungi

Visitor of the Week: Annie Yao

Meet Annie Yao of the University of Connecticut Health Center. Annie is a MD/PhD Candidate in Dr. Riqiang Yan’s lab and recently trained at last month’s Ion Channels in Synaptic and Neural Circuit Physiology course.

Tell us about your research.
My lab studies the pathophysiological underpinnings of Alzheimer’s disease (AD), the most common cause of dementia worldwide. I’m interested in how beta-secretase, an essential enzyme in the development of AD, regulates neuronal excitability and synaptic plasticity.

How did you decide to focus on this area/project?
Since high school, I’ve been interested in combining translational research with helping patients. While working at the Broad Institute/MIT after college in Dr. Guoping Feng’s lab, which studies synapse and brain circuitry dysfunction in neuropsychiatric disorders like autism and schizophrenia, I was inspired to pursue a dual MD/PhD degree with the goal of studying and treating brain disorders. I chose my project after rotating in a couple of labs and being inspired by electrophysiology as a way to study neuronal (dys)function.  

Annie patch-clamp recording of a dendrite in the hippocampal CA1 region.

What and/or who is the inspiration behind your scientific journey?
My parents are first-generation immigrants that moved to the U.S. to pursue their own graduate education in science. The strength and resilience they possessed to make that sort of leap… they have always been an inspiration to me.

What impact do you hope to make through your work?
I hope that my current and future research and clinical training will be able to directly shape patient wellbeing and improve therapeutics.

What do you love most about being a researcher?
One of my favorite parts of being a researcher is that there’s always something more to learn, a new skill to develop, a new perspective to explore.

What drew you to apply to this course?
I have heard amazing things about the Ion Channels course for many years, from both my mentors in previous labs and peers who have taken the course. I knew that this experience would be formative in my growth as a training electrophysiologist. 

What is your key takeaway from the Course; and how do you plan to apply it to your work?
My key takeaway is to be persistent in your troubleshooting of the rig, and as one of our instructors emphasized at the end of our time here, to be rigorous in our practice of electrophysiology and critical about our data.

Patch clamp of a hippocampal CA1 pyramidal neuron while measuring evoked activity.

What feedback or advice would you share with someone considering to participate in this course?
You get out of an experience what you put in! When applying for the course, be sure to emphasize how your specific project would benefit from this immersive experience. When at the course, make use of the expertise that surrounds you every day – you have access to incredible teachers/scientists/electrophysiologists who can help you answer any question you have about your project design or execution. And finally, make time for yourself - 3 weeks of nonstop ephys is gratifying but intense!

What’s the most memorable thing that happened during the Course?
After the last day of hands-on lab work, our cohort went on a sunset sailboat trip together. It felt amazing to be on the water, with beautiful weather and in the company of new friends.

What do you like most about your time at CSHL?
Being surrounded by beautiful nature the second you step outside your cabin or the lab. Cold Spring Harbor is truly such a scenic place to learn in.

Thank you to Annie for being this week's featured visitor. To meet other featured scientists - and discover the wide range of science that takes part in a CSHL meeting or course - go here.

Images provided by Annie Yao

Visitor of the Week: Mohamed "Mo" Gatie

Meet Mohamed “Mo” Gatie of the Sloan Kettering Institute. Mo is a postdoctoral research fellow in Dr. Anna-Katerina Hadjantonakis’ lab. He took part in our 2021 Mouse Engineering Virtual Minicourse and returned last month to train, in-person, at the Mouse Development, Stem Cells & Cancer course last month.

Tell us about your research.
How cells differentiate and acquire specific identities, organize in an orchestrated manner to generate an anatomical, fully functional organ remains an elusive mystery in developmental biology. To understand the mechanisms that govern the formation of endodermal organs, I use high resolution imaging with single-cell genomic technologies, coupled with precision mouse genetics approaches to further our understanding of normal endodermal organ development; a cornerstone for improving the understanding and treatment of congenital birth defects.

How did you decide to focus on this area/project?
During my graduate training, I focused on trying to understand the role metabolism plays in lineage decision using in vitro systems. However, for my postdoctoral work, I wanted to switch gears and study later stages of development and use the mouse as a model organism. I was fortunate enough to be accepted into the lab of Dr. Anna-Katerina Hadjantonakis who is an expert in mammalian embryo development, with a longstanding interest in the endoderm, the tissue which gives rise to the respiratory and digestive tracts and associated organs.

What and/or who is the inspiration behind your scientific journey?
My earliest childhood inspiration would have to be my uncle. I was always amazed by his ability to take things apart, fix them, and put them back together. This really resonated with me, as in developmental biology, we follow a similar process when we’re trying to understand normal development and/or disease.

What impact do you hope to make through your work?
In addition to advancing the field of developmental biology, I would like to provide increased opportunities to marginalized groups, minorities and women in science to remove the barriers these groups face and make science more inclusive and accessible to all.

Where do you see yourself in five years?
I hope to have a paper or two published from my postdoctoral work and focus on my career path towards an independent research program to do science and mentor the next generation of scientists.

What do you love most about being a researcher?
The scientific freedom to explore the unknown. The most satisfying aspect of research is when you first make a discovery--big or small--and for that moment in time you are the only person who knows about it.

What drew you to apply to this course?
The CSHL Mouse Development, Stem Cells and Cancer course is an intensive, 3-week course that brings together world-class researchers, instructors and students to learn and exchange their unique knowledge and expertise. Coming into this with little mouse experience, this course was exactly what I needed to enhance my competence in this area of science. The instructors and TAs were extremely knowledgeable and approachable, and truly made this experience unforgettable.

What is your key takeaway from the course; and how do you plan to apply it to your work?
The course was designed in a way to expose us to many different techniques. The point is not to be an expert, but perhaps learn and develop valuable skills that we can take back to our own labs and implement into our individual research projects.

What feedback or advice would you share with someone considering to participate in this course?
JUST DO IT! The lectures and the technical knowledge and various topics you will learn about during this course are invaluable.

What’s the most memorable thing that happened during the course?
The most memorable aspect is easily the collaboration I established with the CSHL Metabolomics course. I wanted to expand my knowledge and was lucky enough to interact with the students and instructors from the Metabolomics course, which was taking place at the same time as the mouse course. We were able to work on a project together and present the data to the class. Collaboration is exactly what science is all about, and CSHL is the perfect place for this.

Is this your first in-person course/workshop since the pandemic? If so, any thoughts you’d like to share?
Yes, this is my very first workshop ever. To many around the world, this pandemic has been very difficult. We in science thrive on collaboration, networking, discussing ideas as well as going to conferences to learn and present our work. I am extremely grateful for the opportunity to attend the CSHL Mouse Development, Stem Cells and Cancer course to learn from experts in the field and learn from amazing individuals.   

What do you like most about your time at CSHL?
Without a doubt, it is the people I met in the course. I really enjoyed being able to thoroughly plan experiments, talk about science and just spend time at the bar relaxing after a long day with all the wonderful folks. The friendships that I have gained from this course will be everlasting.

Mohamed received a scholarship from the Howard Hughes Medical Institute (HHMI) to cover a portion of his course tuition. On behalf of Mo, thank you to HHMI for supporting and enabling our young scientists to attend a CSHL course where they expand their skills, knowledge, and network.

Thank you to Mo for being this week's featured visitor. To meet other featured scientists - and discover the wide range of science that takes part in a CSHL meeting or course - go here.

Image provided by Mohamed Gatie

Visitor of the Week: Philip Salu

Meet Philip Salu of the North Dakota State University! The Ghanaian citizen is a Ph.D. Candidate and member of Prof. Katie Reindl’s lab. He attended the 2022 course on Metabolomics last month; his first in-person course since the pandemic that he found “frankly refreshing”.

Tell us about your research.
The focus of my research is on drug-resistance development in pancreatic cancer. Our main goal is to better understand the underlying mechanisms using appropriately developed cell line models.

How did you decide to focus on this area/project?
Drug resistance in cancers is a complex phenomenon that is commonly talked about, yet the root cause is not fully understood. There were earlier attempts in my lab at developing specific drug resistance models and so it was a natural thing for me to pick up this project since there is still a lot of discovery to be made.

What and/or who is the inspiration behind your scientific journey?
My scientific journey began with the desire to work in a field where a lot of exciting discoveries have been made knowing that there are even greater prospects ahead. This is of course fueled by my curiosity and open-minded attitude to solving problems.

What impact do you hope to make through your work?
I hope to contribute to scientific knowledge regarding the mechanism of drug resistance development in pancreatic cancer. The disease condition has an abysmal survival rate, therefore, any knowledge that contributes to increased disease susceptibility to therapy will have significant implications for patient survival.  

Where do you see yourself in five years?
Soon, I see myself starting a post-doctoral training to further understand the metabolic needs of cancers and their resistant phenotypes. I would also like to work in a lab that is into biomarker discovery before starting independent research of my own.

What do you love most about being a researcher?
Being a researcher gives me the ability to be open-minded and inquisitive. I derive joy from the successes and channel the failures into an energy of motivation.

What drew you to apply to this course?
I have always wanted to determine changes in metabolic signatures of the cells I work with. So, when I found this course on the CSHL website, I knew I had to take it. 

What is your key takeaway from the Course; and how do you plan to apply it to your work?
The field is very diverse and what you do including procedures and equipment are very relevant in obtaining accurate data. I have come to appreciate the importance in finding ways to use instruments that are available to me in answering basic questions. Also, this course has given me insight into things I should consider when preparing my samples and running them to get consistent data that truly answers my questions.

What feedback or advice would you share with someone considering to participate in this course?
I will highly recommend this course to anyone interested. But more importantly, make connections, friends and share knowledge. The prospects are just boundless.

What’s the most memorable thing that happened during the Course?
The most memorable moment of the course for me was our “Beeromics” experiment. How we sampled different beer brands each night and the effort it took our instructors to run about 200 samples while making sure all other student projects are taken care of amidst instrument breakdowns.

Is this your first in-person course/workshop since the pandemic? If so, any thoughts you’d like to share?
Yes, this is my first in-person course since the pandemic and it was frankly refreshing to have face-to-face interactions again. It's very interesting how in-person meetings are more fruitful compared to online interactions.

What do you like most about your time at CSHL?
My favorite thing was our sailing trip on the 138-year-old Oyster Sloop, Christeen. I really did enjoy how the vessel’s history was told by one of the captains.

Philip received a scholarship from Regeneron to cover a portion of his course tuition. On behalf of Philip, thank you to Regeneron for supporting and enabling our young scientists to attend a CSHL course where they expand their skills, knowledge, and network.

Thank you to Philip for being this week's featured visitor. To meet other featured scientists - and discover the wide range of science that takes part in a CSHL meeting or course - go here.

Image provided by Philip Salu

Visitor of the Week: Kyle McAndrews

Meet Kyle McAndrews of the University of Nebraska Medical Center (UNMC)! Kyle is a 4th year doctoral student in the Cancer Research Graduate Program and a member of Tony Hollingsworth’s lab. He attended our 2022 workshop on Pancreatic Cancer.  

Tell us about your research.
My project is focused on the pancreatic ductal glands, which are a progenitor niche of the main pancreatic duct. We are interested in determining their contribution to pancreatic cancer initiation and progression.

How did you decide to focus on this area/project?
My undergraduate degree was in Wildlife Ecology and Biology and I actually did not plan to become a lab rat, but I was hired as a lab technician in Dr. Sarah Thayer’s laboratory at UNMC, which is where my project began and I became excited about pancreatic cancer research, especially how and where in the pancreas it is initiated.

What and/or who is the inspiration behind your scientific journey?
I first became interested in science when I was a child through my father’s (and later my own) aquarium hobby. I found myself wanting to learn more and more about the natural world. I also had a lot of great science teachers and professors throughout my education that were truly excited about science and encouraged me to continue learning.

What impact do you hope to make through your work?
I hope that my work can help us understand some of the earliest events in the initiating processes of pancreatic cancer. Any knowledge gained surrounding the earliest stages of disease will have important implications for detecting the disease and intervening sooner.

What do you love most about being a researcher?
Being on the cutting edge of what we know is extremely exciting, the seemingly infinite complexity of Biology is mind boggling and being a part of advancing human knowledge is both fulfilling and important.

What drew you to apply to this course?
Some of my lab mates had attended previously and had an amazing experience.

Kyle sectioning frozen tissue sections of mouse pancreas.

What is your key takeaway from the Course; and how do you plan to apply it to your work?
That all of the models which we use to study disease have limitations and that we always need to keep this in mind when making conclusions about data. There are seemingly infinite cell to cell interactions happening at all stages of pancreatic cancer, many of which we are just beginning to scratch the surface. This course has helped broaden my perspective and allowed me to look at my own project from angles that I had never previously considered.

What feedback or advice would you share with someone considering to participate in this course?
Ask questions, make friends and connections to everyone here! There is so much knowledge and experience and having such an intimate setting with leaders in the field is truly priceless.

What’s the most memorable thing that happened during the Course?
Honestly the fact that everything was so casual was a relief. I definitely packed too many polos and pants. Seeing experts in the field from around the country just be who they are and not in their normal professional setting was awesome.

Is this your first in-person course/workshop since the pandemic? If so, any thoughts you’d like to share?
This is my second in-person course/workshop since the pandemic. It was nice to interact with people from outside of my normal routine and setting, which has at times been hard or impossible in the last couple of years.

What do you like most about your time at CSHL?
Playing card games with other attendees after our long days and going for a run down to Lloyd Harbor Road when I could fit it in before dinner was great. As a native Nebraskan I appreciate any second I can spend near salt-water.

Kyle received a scholarship from the Helmsley Charitable Trust to cover a portion of his course tuition. On behalf of Kyle, thank you to the Helmsley Charitable Trust for supporting and enabling our young scientists to attend a CSHL course where they expand their skills, knowledge, and network.

Thank you to Kyle for being this week's featured visitor. To meet other featured scientists - and discover the wide range of science that takes part in a CSHL meeting or course - go here.

Images provided by Kyle McAndrews